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. 2017 Jan 3;114(3):E416–E425. doi: 10.1073/pnas.1612383114

Fig. S3.

Fig. S3.

Western blot images and their corresponding total protein gels showing Kv7.1, minK, Kir2.1, and Cav1.2 expression (arrows) in HL-1 cells transfected or not with Tbx20 WT or p.R311C. As depicted in A and C, the presence of any form of Tbx20 did not modify either Kv7.1 or Kir2.1 protein expression compared with untransfected cells. On the other hand, B displays that both WT and p.R311C Tbx20 increased minK expression in HL-1 cells. Finally, D shows that both WT and p.R311C similarly increased Cav1.2 expression in accordance with the increase in the ICaL density observed in the electrophysiological experiments.