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. 2017 Jan 3;114(3):504–509. doi: 10.1073/pnas.1615072114

Fig. 1.

Fig. 1.

Differential regulation of cargo-receptor endocytosis and TRAIL-induced apoptosis by Dyn1. (A) TRAIL activity (100 ng/mL) in MDA-MB-231 (Upper) and A549 cells (Lower) treated with the indicated siRNAs. (B) FLAG-tag TRAIL uptake or (C) TfnR uptake in MDA-MB-231 (Upper) and A549 cells (Lower). (D) TRAIL activity in A549 WT, Dyn1KO, and Dyn1KO cells reconstituted with Dyn1-EGFP. (E) TRAIL-induced caspase-3/7 activation in parental A549 cells, Dyn1KO and Dyn1KO cells reconstituted with Dyn1-EGFP. (F) FLAG-tag TRAIL uptake in the indicated cell lines. Reduction in cell viability (%) was calculated relative to control wells containing no ligand. All internalization rates were calculated relative to surface-bound TfnR or FLAG-tag TRAIL. All of the results are mean values ± SD (n = 3). Two-tailed Student’s t tests were used to assess statistical significance. *P < 0.05, **P < 0.005, ***P < 0.0005; n.s., nonsignificant.