Fig. S4.

Calcineurin inhibition by FK506 enhances TRAIL-mediated cell killing. Effects of calcineurin inhibition on TRAIL activity in MDA-MB-231 cells (A) or A549 cells (B). Control cells or cells pretreated with FK506 (20 µM) were incubated with the indicated concentrations of TRAIL and assessed for cell viability. Reduction in cell viability by TRAIL (%) was calculated relative to control wells incubated with 0 or 20 µM of FK506. All of the results are mean values ± SD (n = 3). Two-tailed Student’s t tests were used to assess statistical significance. ***P < 0.0005.