Fig. S7.
Validation of IP3R inhibition by XesC and evidence that the effects of RyR inhibition on apoptosis depend on Dyn1 phosphorylation. (A) XesC reduces IP3R-mediated release of calcium induced by bradykinin (BK, 300 nm; gray traces), compared with A549 control (black traces) cells. Results shown mean ± SD from 20 cells per condition. (B) Dyn1 KO A549 cells reconstituted with nonphosphorylatable and constitutively active Dyn1AA (KO + AA) are insensitive to Ry. A549 cells, as indicated, were preincubated with Ry for 30 min, followed by treatment with 100 ng/mL of TRAIL and assayed for cell viability. Reduction in cell viability (%) was calculated relative to control wells containing no ligand. All of the results are mean values ± SD (n = 3). Two-tailed Student’s t tests were used to assess statistical significance. ***P < 0.0005.