Fig. S9.

Caspase-8 inhibition reduces TRAIL induced transient Ca²⁺ spikes and TRAIL endocytosis in MDA-MB-231 cells. (A) Representative TIRFM time-lapse images of MDA-MB-231–expressing GCaMP6f after TRAIL incubation in the presence of caspase-8 inhibitors (10 movies). Boxes illustrate the time course where the Ca²⁺ oscillations occurred, at the locations numbered in the Upper panel. (B) TRAIL or TfnR uptake in control (si-Ctrl) and caspase-8–depleted cells (si-Casp8) and Western blot showing efficiency of caspase-8 depletion. Internalization rates were calculated relative to surface bound TRAIL or TfnR, respectively. All of the results are mean values ± SD (n = 3).