Table 1.
Mutations detected in individual lineages of Min L at the end of P6 (second passage at 39 °C) of the temperature stress test present at ≥45% frequency
| Gene | Nucleotide mutation | Amino acid mutation | Percentage of reads with mutation in indicated lineage no.* | |||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |||
| Intergenic NS2-N | g1123a | / | 85 | |||||||||
| P | a2687u | E114V | 96 | |||||||||
| M | u3798a | N179K | 61 | |||||||||
| SH | c4369a | H22Q | 81 | |||||||||
| SH | c4387g | I28M | 71 | |||||||||
| G | a5384g | E232 (silent) | 47 | |||||||||
| M2-1 | g7823u | A73S | 99 | 93 | 61 | 48 | 83 | 63 | 87 | 57 | ||
| M2-1 | c7870a | N88K | 94 | 96 | ||||||||
| M2-1 | a8013g | E136G | 48 | |||||||||
| L | u10548c† | Y684H† | 97 | |||||||||
| L | u10797c†‡ | S767P†‡ | 82 | |||||||||
| L | g12933a | A1479T | 63 | 85 | ||||||||
| L | a13783c† | Y1762S† | 83 | |||||||||
| 5′ Extragenic (trailer) | u15100c | / | 75 | |||||||||
“/” indicates that the amino acid mutation is not applicable for this particular mutation as the given mutation is localized in a nontranslated region.
*
Percentage of reads with the indicated mutation; only mutations present in ≥45% of the reads are shown. Nucleotide numbering is based on RSV sequence M74568 (biological WT RSV strain A2). Mutations present in ≥5% of reads from this same experiment are shown in Table S1.
†
Mutation involving a codon that had been changed as part of CPD of the L ORF.
‡
Mutation involving a nucleotide position that had been changed as part of CPD of the L ORF.