Fig. 4.
Ranking of MEK1 RASopathy mutations in the Drosophila wing. (A) Ectopic wing veins in flies with overexpressed MEK variants using a low-level ubiquitous driver (Tub-Gal4). Here, classes denote ectopic wing veins at specific locations in the wing. (B and C) Rank of selected MEK mutations based on the number of ectopic wing veins from all classes in females (f) and males (m). The average for each mutation is plotted with the SEM indicated. RASopathies, blue; RASopathies and cancer, green; cancer only, red. Here, NOreR,m = 404, NOreR,f = 335, NWT,m = 69, NWT,f = 59, ND44G,m = 68, ND44G,f = 71, NY130C,m = 70, NY130C,f = 66, NF53S,m = 23, NF53S,f = 85, NF53L,m = 70, NF53L,f = 77, NE203K,m = 42, and NE203K,f = 59. One-way analysis of variance (ANOVA) with Bonferroni correction was used for statistical analysis: *P < 0.1, **P < 0.01, ***P < 0.001. The differences of the following pairs were not statistically significant: (B) Y130C, F53S; Y130C, F53L; F53S, F53L; (C) OreR, WT; WT, D44G; Y130C, F53S; Y130C, F53L; F53S, F53L.