Fig. S1.

Confirmation of molecular cloning of the ZIKV MR766 viral genome into the fission yeast gene-expression pYZ1N and pYZ3N system. (A) Molecular cloning into the pYZ1N gene expression vector. (a) Results of PCR-amplified products of each ZIKV ORF-encoding DNA sequence from ZIKV viral cDNA. (b) Endonuclease restriction digestions of the ZIKV ORF-encoding DNA inserts that were cloned into the pYZ1N. Plasmid DNAs were propagated and isolated from E. coli. (c) Results of yeast colony PCR showing correct inserts of respective ZIKV ORF-carrying plasmids from transformed fission yeast SP223 cells. (B, a–c) Gels for molecular cloning of the ZIKV genome into the pYZ3N vector are shown as in A. The order of ORFs from 1–14: anaC, C, PR, M, prM, E, 2K, NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5. M, molecular marker.