Fig. S4.

Effects of ZIKV proteins on nuclear morphology and cell-cycle regulation. (A) Nuclear morphology. Cells were stained with Hoechst blue fluorescent DNA dye. All cell and nuclear morphologies were examined 45 h after GI. (a) An empty pYZ1N vector control. (b) Structural ZIKV proteins. (c) Nonstructural ZIKV proteins. (B) Cell-cycle profiling with starting cells predominantly in the G2 phase of the cell cycle. All cells were grown in regular EMM medium. The effect of ZIKV protein on the cell cycle was measured by flow cytometry 48 h after GI. (a) Structural ZIKV proteins. (b) Nonstructural ZIKV proteins. (C) Cell-cycle profiling with starting cells predominantly in the G1 phase of the cell cycle. All cells were grown in the low-nitrogen EMM medium to enrich G1 cells (6). The effect of ZIKV protein on cell cycle was measured by flow cytometry 48 h after GI. (a) Structural ZIKV proteins. (b) Nonstructural ZIKV proteins.