Table S1.
Primers used to clone the Zika genes into the pYZ1N fission yeast expression vector
| Gene name | Size, bp | 5′ primer | 3′ primer | Cloning sites |
| anaC | 366 | CTCG CATATG AAAAACCCAAAGAAGAAATCC | CTCG GGATCC TTA TGCCATGGCTGTAGTCAGCAG | NdeI/BamHI |
| C | 312 | CTCG CATATG AAAAACCCAAAGAAGAAATCC | CTCG GGATCC TTA ACGTCTCTTCCTCTCTTTCCTAGC | NdeI/BamHI |
| Pr | 279 | CTCG CATATG GCAGAGATCACTAGACGCGGG | CTCG GGATCC TTA TCTTCTAGATCGCCGTGCCTC | NdeI/BamHI |
| M | 225 | CTCG CATATG GCCGTGACGCTCCCTTCTC | CTCG GGATCC TTA ACTGTATGCCGGGGCAATC | NdeI/BamHI |
| prM | 504 | CTCG CATATG GCAGAGATCACTAGACGCGGG | CTCG GGATCC TTA ACTGTATGCCGGGGCAATC | NdeI/BamHI |
| E | 1,512 | CTCG CATATG ATCAGGTGCATTGGAGTCAGC | CTCG GGATCC TTA AGCAGAAACAGCCGTGGAGAG | NdeI/BamHI |
| 2K | 69 | CTCG CATATG TCTCCCCAAGATAACCAGATGG | CTCG GGATCC TTA TGCAGTTATCAAACCTAGAAGGC | NdeI/BamHI |
| NS1 | 1,056 | CTCG CATATG GACGTGGGGTGCTCAGTGGAC | CTCG GGATCC TTA CGCTGTCACCATTGACCTCAC | NdeI/BamHI |
| NS2A | 678 | CTCG GGATCC ATG GGGTCAACCGATCATATGGAC | CTGTC GCGGCCGC TTA CCGCTTCCCACTCCTTGTG | BamHI/NotI |
| NS2B | 390 | CTCG CATATG AGCTGGCCCCCTAGTGAAG | CTCG GGATCC TTA CCTTTTCCCAGTCTTCACATACAC | NdeI/BamHI |
| NS3 | 1,851 | CTCG CATATG AGTGGCGCCCTCTGGGAC | CTCG GGATCC TTA TCTTTTTCCAGCGGCGAATT | NdeI/BamHI |
| NS4A | 381 | CTCG CATATG GGAGCGGCTTTGGGAGTAAT | CTCG GGATCC TTA TCTTTGCTTCTCTGGCTCGG | NdeI/BamHI |
| NS4B | 753 | CTCG CATATG AACGAACTTGGATGGCTGG | CTCG GGATCC TTA ACGTCTCTTAACCAGGCCAG | NdeI/BamHI |
| NS5 | 2,709 | CTCG GTCGAC ATG GGAGGTGGGACGGGAGAGAC | CTGTC GCGGCCGC TTA CAACACTCCGGGTGTGGACC | SalI/NotI |
Restriction enzymatic sites are underlined.