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. 2016 Dec 14;25(2):216–221. doi: 10.1038/ejhg.2016.145

Figure 1.

Figure 1

Expression of SIK1 wild-type and mutant proteins in human fetal neurons. (a) The lentiviral vector pCDH-GFP-Puro was used with a V5-epitope tag inserted in-frame at the amino terminal of the SIK1 cDNA. Vector controls included pCDH-GFP-Puro without insert or expressing the nuclear envelope protein lamin A. (b) Western blot of V5-tagged, wild-type and mutant SIK1 proteins in primary human neurons derived from the cortex at 20 weeks of gestation. (c–e) Example of lentiviral transduction followed by puromycin selection of primary human neurons (c) with SIK1 p.(Gln633*) resulted in robust V5 (d) and GFP staining (e). Bar=400 μm.