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Expression of DPT and SEMA3D genes in the human cochlea and semicircular canals (sc) by qPCR. (a) Validation of the expression using primers for DPT (250 bp), SEMA3D (213 bp) and a housekeeping gene HPRT1 (92 bp) in agarose gel. Each sample has a technical replication. (b) ΔCt values of DPT (ΔCt cochlea=3.85±0.3; ΔCt sc=2.42±0.27) and ΔCt values of SEMA3D (ΔCt cochlea=0.56±0.16; ΔCt sc=−0.55±0.13).
