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. 2017 Jan 23;7:40983. doi: 10.1038/srep40983

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(a,b) The efficiency of four different sh-RNA sequences (sh-RNA1, 2, 3, 4) targeting syncytin-1 and negative sh-RNA sequence which was used as negative control by qRT-PCR and western blot. (c) The number of fused cells between SCC-9 and HUVEC when syncytin-1 in SCC-9 was knocked down by sh-RNA by number counting. (d) The rate of cell fusion between SCC-9 and HUVEC measured by FACS. When co-cultured SCC-9 whose syncytin-1 was knocked down by sh-RNA with HUVEC, the fusion rate could be 0.196 ± 0.008% compared to the negative control group, 0.768 ± 0.012%. (e) The statistical analysis of FACS.