Skip to main content
. 2017 Jan 23;7:40981. doi: 10.1038/srep40981

Figure 6. Differential role of Estrogen receptors in NF-κB and AP-1 transcription activity.

Figure 6

SEAP expression of MCF7 cells transfected with either the pNifty2-SEAP (a and b) or the pNifty3-A-SEAP (c) reporter plasmid. (a and c) Transfected MCF7 cells were pre-incubated with ER agonists PPT, DPN or G1 (0, 10, 100 and 1000 nM) for 24 h and then stimulated with 100 ng/ml of flagellin. (b) Effect of the ER blockade by specific ER antagonists (MPP, PHTPP or G15). A 2 × 2 factorial experimental design was used where transfected MCF7 cells were either: (1) cultured in the absence of both ER antagonist and E2 (control); (2) treated with 1 μM of ER antagonist for 30 min; (2) pre-incubated with 10 nM E2 for 24 h or (2) treated with 1 μM of ER antagonist for 30 min and then pre-incubated with 10 nM E2 for 24 h. The cells were then stimulated with 100 ng/ml of flagellin and analyzed at 24 h using NovaBrightTM Secreted Placental Alkaline Phosphatase (SEAP) Enzyme Reporter Gene Chemiluminescent Detection System 2.0. Control Data of NF-kB activity are reported as the fold induction of SEAP activity over untreated controls. Data are representative of at least five independent experiments. Error bars denote SEM. Different letters mean significant difference (p < 0.05).