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. 2017 Jan 23;7:40979. doi: 10.1038/srep40979

Figure 6. Knockdown of CCNI2 decreases cell cycle progression and cell proliferation of HeLa cells.

Figure 6

The specificity and efficiency of siRNAs against CCNI (A) and CCNI2 (B) were examined by quantitative PCR. HeLa cells were transfected with siRNAs at day 0 and day 1, and the expression level of CCNI or CCNI2 was determined by qPCR analysis at day 2. (C) Cell cycle profiles of CCNI-depleted and CCNI2-depleted HeLa cells. (D) BrdU incorporation of CCNI-depleted and CCNI2-depleted HeLa cells. (E) Cell proliferation of CCNI-depleted and CCNI2-depleted HeLa cells was examined by MTT assay. The bar graphs and the table show quantification of the results, with each value represents the mean ± SD of three independent experiments. Statistical significance is shown using the Student t test analysis; *P < 0.05; **P < 0.01; ***P < 0.001.