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. 2017 Jan 23;7:40764. doi: 10.1038/srep40764

Figure 2. Only 4R-Tau expression during development impact external adult eye phenotype.

Figure 2

(A) Drosophila external eye phenotype upon 3R or 4R-Tau expression in retinas during development using the GMR-Gal4 driver (Scale bar = 100 μm). (B) Western blot from adult fly heads showing the presence of Tau protein isoforms upon induction of transgene expression using the GMR-Gal4 driver. (C) Quantification of eye size upon 3R or 4R-Tau expression using the GMR-Gal4 driver (n = 10 fly eyes, mean ± SD). (D) Drosophila external eye phenotype upon inhibition (18 °C) of 4R-Tau expression in retinas during development (Scale bar = 100 μm). (E) Adult photoreceptors in living flies imaged using a rh1-GFP transgene at 4 weeks after continuous inhibition (18 °C) or adult-onset induction of 4R-Tau expression (29 °C) (Scale bar = 10 μm). (F) Western blot from adult heads showing the level of 4R-Tau isoform after induction in the three genotypes studied. The corresponding full-length western blot is presented in Sup Fig. 5A. (H) Quantification of photoreceptor neurons at 4 weeks showing no loss of photoreceptor neurons upon adult-onset induction of 4R-Tau expression (n = 6 fly eyes, mean ± SD).