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. 2017 Jan 23;7:40940. doi: 10.1038/srep40940

Figure 5. The intracellular Ca2+ of MLO-Y4 was fluorescent labeled by Fluo-3 AM and recorded for 30 min after the DIA treatment.

Figure 5

In normal medium (+Ca2+), the intracellular Ca2+ signal attenuated with time owing to the photobleaching effect (a), After the DIA treatment, the Ca2+ signal increased although the photobleaching still exist. (b) While in the Ca2+ free medium (−Ca2+), the DIA induced Ca2+ increase disappeared and displayed a downward trend along with time (d).