Table 2.
Summary of antibody dilutions and conditions used in the immunoblotting analysis of mitochondrial dynamics, mitochondrial biogenesis and synaptic proteins
| Marker | Primary antibody—species and dilution | Purchased from (company, location) | Secondary antibody, dilution | Purchased from (company, location) |
|---|---|---|---|---|
| Drp1 | Rabbit polyclonal 1:500 | Novus Biological, Littleton, Colorado, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| Fis1 | Rabbit polyclonal 1:500 | MBL International Corporation-life. Woburn, Massachusetts, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| Mfn1 | Rabbit polyclonal 1:400 | Novus Biological, Littleton, Colorado, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| Mfn2 | Rabbit polyclonal 1:400 | Abcam, Cambridge, Massachusetts, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| Opa1 | Rabbit polyclonal 1:500 | Novus Biological, Littleton, Colorado, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| CypD | Mouse monoclonal 1:500 | EMD, Calobiochem Chemicals INC, Gibbstown, New Jersey, USA | Sheep anti-mouse HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| SYN | Rabbit polyclonal 1:400 | Abcam, Cambridge, Massachusetts, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| PGC1α | Rabbit polyclonal 0.5 µg/mL | Novus Biological, Littleton, Colorado, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| Nrf1 | Rabbit monoclonal 1:1000 | Abcam, Cambridge, Massachusetts, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| Nrf2 | Rabbit polyclonal 1:500 | Novus Biological, Littleton, Colorado, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| TFAM | Rabbit polyclonal 1:1000 | Abcam, Cambridge, Massachusetts, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| PSD95 | Rabbit monoclonal 1:300 | Abcam, Cambridge, Massachusetts, USA | Donkey anti-rabbit HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |
| B-actin | Mouse monoclonal 1:500 | Sigma-Aldrich, St Louis, Missouri, USA | Sheep anti-mouse HRP 1:10,000 | GE Healthcare Amersham, Piscataway, New Jersey, USA |