Table 3.

Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of mitochondrial dynamics, mitochondrial biogenesis and synaptic proteins

Marker	Primary antibody—species and dilution	Purchased from (company, location)	Secondary antibody, dilution, Alexa fluor dye	Purchased from (company, location)
Drp1	Rabbit polyclonal 1:300	Novus Biological, Littleton, Colorado, USA	Donkey anti-rabbit IgG Alexa Fluor 488 conjugate	Thermo Fisher Scientific, Waltham, Massachusetts, USA
Fis1	Rabbit polyclonal 1:300	Protein Tech Group, Inc, Chicago, Illinois, USA	Donkey anti-rabbit IgG Alexa Fluor 488 conjugate	Thermo Fisher Scientific, Waltham, Massachusetts, USA
Mfn1	Rabbit polyclonal 1:300	Protein Tech Group, Inc, Chicago, Illinois, USA	Donkey anti-rabbit IgG Alexa Fluor 488 conjugate	Thermo Fisher Scientific, Waltham, Massachusetts, USA
Mfn2	Rabbit polyclonal 1:200	Protein Tech Group, Inc, Chicago, Illinois, USA	Donkey anti-rabbit IgG Alexa Fluor 488 conjugate	Thermo Fisher Scientific, Waltham, Massachusetts, USA
OPA1	Rabbit polyclonal 1:500	Novus Biological, Littleton, Colorado, USA	Donkey anti-rabbit IgG Alexa Fluor 488 conjugate	Thermo Fisher Scientific, Waltham, Massachusetts, USA
SYN	Rabbit polyclonal 1:200	Protein Tech Group, Inc, Chicago, Illinois, USA	Donkey anti-rabbit IgG Alexa Fluor 488 conjugate	Thermo Fisher Scientific, Waltham, Massachusetts, USA
PSD95	Rabbit polyclonal 1:400	Protein Tech Group, Inc, Chicago, Illinois, USA	Donkey anti-rabbit IgG Alexa Fluor 488 conjugate	Thermo Fisher Scientific, Waltham, Massachusetts, USA