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. 2017 Jan 26;2(2):e91377. doi: 10.1172/jci.insight.91377

Figure 1. Pulmospheres are 3D structures made of multiple cell types and extracellular matrix.

Figure 1

(A) Schematic representation of pulmosphere preparation. Representative phase-contrast microscopic image. H&E-stained pulmosphere. Scale bar: 250 μm. (B) Immunofluorescent staining of pulmospheres from IPF patients for α-SMA, SPC, CD31, CD11b, fibronectin-EDA, collagen type I, and collagen type IV in paraffin-embedded sections. All immunofluorescent slides were costained with DAPI for nuclei. Five random pulmospheres were sampled from each control subject (n = 9). Scale bar: 250 μm. (C) Immunofluorescent staining of pulmospheres from IPF patients for α-SMA, SPC, CD31, CD11b, fibronectin-EDA, collagen type I, and collagen type IV in paraffin-embedded sections. All immunofluorescent slides were costained with DAPI for nuclei. Five random pulmospheres were sampled from each IPF patient (n = 20). Scale bar: 250 μm.