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. 2017 Jan 26;2(2):e91377. doi: 10.1172/jci.insight.91377

Figure 5. Differential invasiveness of pulmospheres from patients with IPF following exposure to nintedanib and pirfenidone.

Figure 5

(A) Measurement of fold change of zone of invasion (ZOI) was calculated as the ratio of ZOI% with treatment to ZOI% without treatment in vitro with antifibrotic drug. (B) Median values of ZOI >1 represent pulmospheres nonresponsive to the treatment, and median values of ZOI <1 represent pulmospheres responsive to drug treatment. For each patient tested, 5 pulmospheres were seeded without either test drug; 5 pulmospheres were seeded with nintedanib (1 μM); and 5 pulmospheres were seeded with pirfenidone (1 mM). ZOI fold change values were obtained from the ratio of ZOI% with treatment to ZOI% without treatment of pulmospheres in vitro with antifibrotic drug for each patient. Box-and-whisker plots for IPF patient pulmospheres treated with (C) nintedanib and (D) pirfenidone. Whiskers show maximum to minimum values. Lines in boxes represent median values. The “+’”signs inside boxes represent the mean values.