Figure 3.

Acetazolamide (AZ) effects on medial prefrontal cortical (mPFC) firing combined net inhibition with increased paired‐pulse responsivity in a minor portion of neurons. (A) Top: heat plot with single units (i.e., their nonperievent rate histograms) along dimension 1 (Y) and time bins (3 min width) in dimension 2 (X). Spike counts were Z‐scored (color bar) against the initial 15 min baseline period. Black triangles represent injections. Bottom: Mean ± standard error from the heat‐plot data (n = 22). The asterisk indicates an effect of time, after one‐way repeated measures ANOVA. (B) Two representative neurons: their perievent raster plots (top), and correspondent 3 msec‐binned histograms (bottom). Red triangles situate paired‐pulse stimuli, and post‐AZ firing rates are from the initial 30 min post‐AZ period. A subjacent voltage trace from Figure 2 (left histogram) illustrates the timing between field and unit responses to hippocampal stimuli. (C) Top: firing responses to conditioning (Resp1) and test pulses (Resp2) after counting spikes within 15–42 poststimulus latencies. Bottom: ratios between Resp2 and Resp1 firing rates. The asterisk informs an effect of time, after one‐way repeated measures ANOVA. Data were averaged across four periods: baseline, post‐Veh, initial 30 min post‐AZ, and final 30 min post‐AZ. All data are shown as mean ± standard error.