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. 2016 Nov 9;65(2):105–116. doi: 10.1369/0022155416677035

Figure 3.

Figure 3.

Histochemical and epifluorescence tests in stems in secondary growth of Nicotiana tabacum. T1: Hand-sectioned fresh material submitted to the histochemical reactions. T2: Material fixed in Karnovsky’s solution, embedded in PEG, sectioned, and submitted to the histochemical reactions. T6: Material fixed in Karnovsky’s solution, embedded in PEG, sectioned, and stained. Violet, Blue, Green: Epifluorescence microscopy photographs were taken under violet, blue, or green light (indicated in each image). Scale bars: 50 µm. Abbreviations: PEG, Polyethylene glycol; fb, xylem fibers; ra, radial parenchyma; ve, vessel element; vc, vascular cambium.