Table 3.
Summary of Results After Each Treatment (T1, T2, T3, T4, T5, and T6).
| Staining | T1 | T2 | T3 | T4 | T5 | T6 |
|---|---|---|---|---|---|---|
| Blank sections | NA | NA | + (*) | NA | NA | + |
| Ferric chloride | + | − | NA | + (*) | + (*) | NA |
| Vanillin/HCl | + | − | NA | − | − | NA |
| DMACA | + | − | NA | + (*) | + (*) | NA |
| Wiesner’s test | + | ± | NA | NA | NA | + |
| Maule’s test | + | + (*) | NA | NA | NA | NA |
| Safranin/astra blue | + | + | NA | NA | NA | + (*) |
| Basic fuchsin/astra blue | + | + | NA | NA | NA | + |
| Sudan black B | + | + | NA | NA | NA | NA |
Results: (+) positive; (−) false negative; (±) sometimes false negatives; (*) best treatments for detection of each phenolic derivative; (NA) not applicable. Abbreviation: DMACA, p-dimethylamino-cinnamaldehyde.