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. 2017 Jan 23;7:40021. doi: 10.1038/srep40021

Table 3. Results of the indirect ELISAs performed with the squid hemocyanin samples against IgE and IgG.

Pressure (MPa) IgE binding*
 IgG binding#
P1 P2 P3 P4 P5 R RSGF RSIF
0.1 1.35 ± 0.05a 0.81 ± 0.02a 0.77 ± 0.03a 0.53 ± 0.05a 0.44 ± 0.03a 1.37 ± 0.06a 1.20 ± 0.07a 0.50 ± 0.03a
200 1.07 ± 0.05b 0.50 ± 0.02b 0.47 ± 0.03b 0.29 ± 0.05b 0.27 ± 0.04b 1.17 ± 0.05b 0.91 ± 0.03b 0.33 ± 0.02b
400 0.93 ± 0.02c 0.31 ± 0.02c 0.30 ± 0.03c 0.22 ± 0.01c 0.21 ± 0.01c 1.06 ± 0.03c 0.83 ± 0.02c 0.22 ± 0.03c
600 0.93 ± 0.03c 0.32 ± 0.03c 0.31 ± 0.02c 0.21 ± 0.02c 0.21 ± 0.01c 1.06 ± 0.02c 0.83 ± 0.02c 0.27 ± 0.02c

Values were expressed as mean ± standard deviation (SD) (n = 3); means in the same column with different lowercase letters (a–c) are significantly different (p < 0.05).

*P1–P5: indirect ELISAs performed with the human sera of five allergic patients.

#R: indirect ELISA performed with rabbit anti-squid hemocyanin polyclonal antibodies. RSGF: indirect ELISA performed with rabbit anti-squid hemocyanin polyclonal antibodies for hydrolysates after the simulated gastric fluid (SGF) digestion. R-SIF: indirect ELISA performed with rabbit anti-squid hemocyanin polyclonal antibodies for hydrolysates after the simulated intestinal fluid (SIF) digestion.