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. 2017 Jan 23;7:41335. doi: 10.1038/srep41335

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Endometrial CD3+CD8+ T cells from 10 controls (•) or 10 RM (Δ) were stained with antibodies CD3-PeCy5, CD8-PECy7, CD45RO-APCCy7, CD103-FITC, CD69-PE with FMO controls. The proportion of cells expressing CD45RO (a) and CD103 and CD69 expression was determined on CD45R0−’ve (b) and CD45RO+ ’ve (c) CD3+/CD8+ T cell populations. Representative example of flow cytometry staining profile of CD103 and CD69 on control (d) and RM (e) CD8+CD45RO+ cells. CD69 expression on CD8+CD45RO+ cells in RM women versus time since last miscarriage (weeks) (f) and showing CD8 and CD69 correlative expression (g). The mean fluorescence intensity of the CD4 and CD8 co-receptor on samples was also calculated (h), representative flow cytometry images of CD8 and CD4 staining on CD3-T cells from control (i) and RM (j) women. Bars represent mean+/− S.E.M. For significance values a Student’s t-test comparison of controls and RM was performed.