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. 2017 Jan 23;7:41327. doi: 10.1038/srep41327

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Optical microscopy images of trans-azoTAB:PAA microparticles (i) before and (ii) during UV-light irradiation to produce single spherical particles of cis-azoTAB:PAA; insets show particle highlighted by the arrow under polarized light indicating loss of birefringence after UV exposure; scale bars = 10 μm. (b) 3D AFM imaging of a single air-dried trans-azoTAB:PAA microparticle viewed (i) in the dark, and (ii) under in situ UV irradiation and transformation to cis-azoTAB:PAA, showing photo-induced loss of surface texture. (c) Time-lapse series of video images showing UV-induced fragmentation and division of a single trans/cis-azoTAB:PAA multipodal structure into a series of spherical particles of cis-azoTAB:PAA within 1 s. Arrows highlight localised pinching events; numbers correspond to spherical particles produced by fragmentation of marked filaments; scale bars = 5 μm. (d) (i) Confocal fluorescence microscopy images of a single azoTAB:PAA microparticle containing Cy5-ssDNA during blue/UV light-induced reconfiguration. Arrows highlight the position of the initial particle and products; scale bars = 5 μm. (ii) Corresponding fluorescence intensity profiles recorded along the dotted lines shown in (i).