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. 2017 Jan 23;7:41282. doi: 10.1038/srep41282

Figure 3. Effects of AVG and ethephon treatments on the attractiveness of soybean root to Heterodera glycines.

Figure 3

The roots of 12-day-old soybean seedlings pretreated with water (Control), 50 μM AVG, or 200 μM ETH for 24 h were excised and placed into Pluronic F-127 gel containing 200 J2. (a) Microscopic observation of nematodes and root tips at 2 h after assay start. (b) The number of nematodes touching the root within the terminal 5 mm at 2 h after assay start. (c) Soybean roots stained with acid fuchsin, which stains nematodes red, at 6 h after assay start. (d) The number of stained nematodes inside the root at 6 h after assay start. Bars are the mean ± SE from one representative experiment (n = 24). The experiments were repeated three times with similar results. Bars with different letters indicate significant differences (P < 0.05, Tukey’s t Test). Scale bar = 200 μm.