Figure 5. Effects of chemical modulation of ethylene levels or signaling on the attractiveness of Arabidopsis roots to Heterodera glycines.

The roots of 7-day-old Arabidopsis seedlings (Col-0) pretreated with water (Control), 200 μM ethephon (ETH), 10 μM AgNO₃ or 50 μM AVG for 24 h were excised and placed into Pluronic F-127 gel containing 300 J2. Nematodes and root tips of Arabidopsis at 2 h after assay start (a). The number of nematodes touching the root within the terminal 5 mm was counted (b) at 2 h after assay start. Bars are the mean ± SE from one representative experiment (n = 24). These experiments were repeated three times with similar results. Bars with different letters indicate significant differences (P < 0.05, Tukey’s t Test). Scale bar = 500 μm.