Figure 9. Attraction of Heterodera glycines to the root tips of ethylene-insensitive Arabidopsis mutants.

(a) Representative images showing the response of nematodes to Arabidopsis mutants defective in positive regulation of the ET signaling pathway. Images were taken 2 h after placing seedlings in 1 ml of Pluronic F-127 gel containing 300 J2. Scale bar = 200 μm. (b) The number of nematodes touching the terminal 5 mm of the root was counted at 2 h after assay start. Bars represent mean ± SE of one representative experiment (n = 24). Asterisks represent significant differences compared to wild-type Arabidopsis (Col-0 or Ler) using Student’s t-test (*P < 0.05). The experiments were repeated three times with similar results.