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. 2016 Jul 8;66(1):124–136. doi: 10.1136/gutjnl-2016-312078

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Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

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CD11b⁺ myeloid cells are required for oncogenic Kras-driven pancreatic intraepithelial neoplasia (PanIN) formation. (A) Genetic makeup of the iKras*;CD11b- diphtheria toxin receptor (DTR) (p48Cre;TetO-Kras^G12D; Rosa26^{rtTa-IRES-EGFP};CD11b-DTR) and iKras*;p53*;CD11b-DTR (p48Cre;TetO-Kras^G12D; Rosa26^{rtTa-IRES-EGFP};p53^R172H/+;CD11b-DTR) mouse models is shown. (B) Experimental design is shown. Doxycycline water was given to iKras* and iKras*;CD11b-DTR mice to induce Kras* expression for 3 days before 2 consecutive days of intraperitoneal caerulein injections to induce pancreatitis. Mice also received diphtheria toxin (DT) injection to deplete CD11b⁺ myeloid cells 1 day prior to pancreatitis induction; n=4–8 mice per time point. (C) H&E and (D) phosph-ERK1/2 staining of wild-type control, CD11b-DTR, iKras* and iKras*;CD11b-DTR pancreas 1 day and 1 week post-pancreatitis induction. Scale bar 50 μm.