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. 2016 Jul 13;66(1):59–69. doi: 10.1136/gutjnl-2015-310874

Figure 3.

Figure 3

Low-dose thioguanine (TG) improved chronic dextran sodium sulfate (DSS)-induced colitis in hypoxanthine (guanine) phosphoribosyltransferase (Hprt)−/− mice. Hprt−/− mice were treated with ±DSS 0.5% in drinking water for four cycles, ±daily gavaged TG 0.5 mg/kg for the last two cycles (28 days from D25): (A) Disease activity indices (DAIs) two-way ANOVA p<0.05, p<0.001 from day 42; (B) colon weight/length ratio; (C) histological colitis scores for proximal colon (PC), mid-colon (MC) and distal colon (DC) in Hprt−/− treated with or without DSS or TG; (D) representative H&E from MC of Hprt−/− treated with 0.5% DSS, ±TG 0.5 mg/kg; (E) peripheral blood white blood cell (WBC) counts and (F) mesenteric lymph node (MLN) total cell number. Statistical analysis: Mann-Whitney non-parametric test. Symbols: *versus Hprt−/− TG 0 mg/kg; #versus Hprt−/−DSS TG 0 mg/kg. Scale bar=100 μm.