Fig. 3.

Cross reactive mAbs were used to validate specificity of HAB1A and HAL36A, a rat anti-mouse CD4 (GK1.5 IgG2b, (Dialynas et al., 1983)) cross reactive with hamster CD4 and mouse anti-rat CD8β 341 (IgG2a) specific for mouse CD8 (Hammerbeck and Hooper, 2011) showed HAB1A and HA36A recognize the same molecule (labelling only shown for HAL36A, Fig. 3A). Comparison with mAb 341 (mouse anti-rat CD8β) showed HAB1A labels a subset distinct from CD8 (Fig. 3B). Multiple hamsters were used in the UK and the US while validating cross reactivity and specificity.