Table 1.
Monoclonal antibodies (WSU Monoclonal Antibody Centre) and Specificities.
| mAb | Ig isotype | Putative specificity1 | Specificity and% of cells2 |
|---|---|---|---|
| H42A | IgG2a | MHC II | MHC II, 48% |
| BAQ30A | IgG1 | CD18 | CD18, 100% |
| HAL4A | IgG3 | MHC class II | MHC class II, 50% |
| HAL16A | IgG1 | MHC class II | MHC class II, 50% |
| HAB2A | IgG1 | T | 33%–43% (CD4 included) |
| HAL26A | IgG1 | T | 42%–63% (CD4 included) |
| HAT19A | G2a | T | 39–53% (CD4 included) |
| HAT24A | IgG1 | T | 53%–73% |
| HAB1A | IgG1 | T subpopulation | 12–44% (CD4) |
| HAL36A | IgG2a | T subpopulation | 16–42% (CD4) |
| HAL9A | IgG1 | B | n.d. |
| HAL11A | IgG1 | B | n.d. |
| HAL14A | IgG2b | B | B 23% |
| HAL17A | IgG2a | B | n.d. |
| HASA7A | IgG1 | B | 34–49% not CD4 |
| HAB6B | IgG2a | Pan lymphocyte (+monocyte subset?) |
n.d. |
| HASA18A | IgM | Pan lymphocyte (+monocyte subset?) |
n.d. |
| HASA25A | IgG1 | CD45 predicted | n.d. |
| HAT13A | IgG2b | CD45 predicted | CD45 |
| HAT7A | IgG2a | CD44 predicted | n.d. |
| HAT16A | IgG2b | CD11a predicted | n.d. |
| HAB3A | IgG1 | Pan leukocyte | >95% incl CD4 and CD? |
| HASA26B | IgG1 | Granulocyte + | n.d. |
Legend:
Based on labelling characteristics of lymphocyte, monocyte and granulocyte-enriched fractions of whole blood leukocytes and 2-colour FC comparison with MHC class II positive and negative fractions of the leukocytes.
Proposed specificities based on two colour comparisons of MNC (PBMC or spleen/MLN MNC) labelling by the mAbs compared to each other and a defined CD-specific mAb (GK1.5 anti-CD4). For the frequency ranges of the phenotyped cells, six different MNC samples from different hamsters were used for the analyses, but not all antibodies were tested at the same time (n = 3 or 4). This is why the% frequencies of HAB2A, HAL 26A and HAT19A have different ranges, in spite of recognising the same molecule. Nd = not determined. These mabs are listed as they are available for further characterisation by the research community.