Table I.

LC/MS/MS Assay Within-Day and Between-Day Precision and Accurate Data of S-AR-42 in Mouse Plasma, Mouse Urine, Mouse Bone Marrow, and Rat Plasma

Matrix type	Nominal concentration, μM (ng/mL)	Within-day			Between-day
		Observed concentration, μM (ng/mL)	CV (%)	Accuracy (%)	Observed concentration, μM (ng/mL)	CV (%)	Accuracy (%)
Mouse plasma	0.016 (5)	0.016 ± 0.002 (5.0 ± 0.5)	9.5	99.4	0.016 ± 0.001 (5.1 ± 0.5)	8.8	102.5
	0.16 (50)	0.15 ± 0.01 (45.6 ± 3.0)	6.6	91.3	0.16 ± 0.00 (50.1 ± 0.3)	0.6	100.2
	1.6 (500)	1.51 ± 0.08 (471 ± 24)	5.0	94.2	1.61 ± 0.02 (503 ± 7)	1.4	100.7
Mouse urine	0.016 (5)	0.016 ± 0.001 (4.8 ± 0.2)	4.6	95.2	0.015 ± 0.002 (4.72 ± 0.46)	9.8	94.3
	0.16 (50)	0.16 ± 0.01 (49.9 ± 6.0)	5.3	98.3	0.16 ± 0.02 (48.4 ± 5.1)	10.6	96.9
	1.6 (500)	1.46 ± 0.02 (454 ± 7)	1.5	90.9	1.47 ± 0.06 (460 ± 20)	4.4	92.0
Mouse bone marrow	0.016 (5)	0.016 ± 0.001 (5.1 ± 0.4)	8.3	102.2	0.015 ± 0.001 (4.8 ± 0.4)	7.6	95.3
	0.16 (50)	0.16 ± 0.01 (49.9 ± 1.8)	3.5	99.9	0.17 ± 0.01 (52.2 ± 2.4)	4.6	104.3
	1.6 (500)	1.66 ± 0.04 (518 ± 14)	2.7	103.7	1.59 ± 0.09 (498 ± 29)	5.8	99.6
Rat plasma	0.016 (5)	0.016 ± 0.002 (5.0 ± 0.7)	13.2	99.2	0.016 ± 0.002 (4.9 ± 0.5)	9.7	97.7
	0.16 (50)	0.16 ± 0.01 (51.3 ± 2.6)	5.2	102.6	0.16 ± 0.00 (49.3 ± 0.7)	1.5	98.7
	1.6 (500)	1.55 ± 0.05 (483 ± 15)	3.0	96.6	1.60 ± 0.01 (502 ± 3)	0.7	100.6

Within-day data are n = 6 (plasma and urine) or n = 4 (bone marrow), and between-day data are n = 18 (plasma and urine) or n = 12 (bone marrow). Data are expressed as mean ± SD