Fig 4. Initial orientation of actin filaments regulates bundle formation and tension generation.
Densities of motors (RM) and ACPs (RACP) used in cases shown here are 0.08 and 0.01, respectively. (a-c) (1st column) Orientations where barbed ends of actin filaments in networks are initially directed. Red on the circles located at the bottom-right corner represents the range of the orientation. Arrows in the boxes represent examples of filaments with corresponding initial orientations. (2nd, 3rd, 4th columns) Snapshots showing actin density in the networks at t = 10, 30, and 60 s with initial orientation indicated in the 1st column. (5th column) Initial and final orientations of actin filaments. Final orientation indicates orientation of filaments measured at a time point when compaction time is defined. (d) Time evolution of tension for cases with biased initial orientations shown in (a-c) and isotropic initial orientation. (e) Number of buckling events occurring during simulations for cases shown in (d). (f) Time evolution of a fraction of antiparallel filament pairs for cases shown in (d).