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. 2017 Jan 23;12(1):e0170552. doi: 10.1371/journal.pone.0170552

Fig 7. TthCsm-mediated DNA cleavage does not require RNA cleavage.

Fig 7

(A) TthCsm-mediated cleavage of a complementary ssRNA either with (deoxy-RNA) or without (RNA) deoxynucleotides adjacent to the regularly spaced cleavage sites was tested and analyzed by denaturing PAGE, as in Fig 5C. (B) ssDNA cleavage by TthCsm was assayed as in Fig 5B, but with the RNA substrates in (A). (C) The wild-type TthCsm (WT) or TthCsm containing a D34A or D34N mutation in the Csm3 subunit (D34A, D34N) were tested for cleavage of a complementary ssRNA target, as in (A). (D) WT, D34A, or D34N TthCsm complexes were tested for ssDNA cleavage as in (B).