Fig 3. Illustration of intermolecular interactions governed by NOD1 and RIP2 CARDs.

(A) All possible intermolecular interactions governed by type-Ia interface of NOD1^CARD and type-Ib interface of RIP2^CARD (acquired from S2 Fig) are presented in left panel and the corresponding dynamic distances (both atomic and residual) of the mentioned interacting pairs are outlined in right panel. (B) Structure comparison of NOD1-RIP2 CARD-CARD complex with solved type-I heterodimers; left panel: with RIG-I-MAVS (4P4H); middle: Ap1-C9 CARD-CARD complex (3YGS); right panel: superimposed view of final snapshots of NOD1-RIP2 CARD-CARD complexes (obtained from three individual production runs). The detailed type-I mode of molecular interactions of respective complexes were presented in lower panel. The critical interacting residues (involved in respective CARD-CARD interactions; experimental/ predicted (this study)) were potrayed in ball-stick model and the residues were coloured based on their physicochemical properties.