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. Author manuscript; available in PMC: 2017 Jan 23.
Published in final edited form as: Clin Transplant. 2016 Aug 8;30(9):1115–1133. doi: 10.1111/ctr.12795

Figure 2.

Figure 2

Validation of eight selected genes by quantitative real-time PCR. Each dot represents one biopsy. Gene expression is represented as ΔCt after normalizing to the housekeeping gene GAPDH. Validation was performed on all 33 samples analyzed by NanoString as well as 22 additional biopsies. P values were calculated using a Student’s t test and are shown in Table 7. ns, not significant, *P<.05, **P<.01, ***P<.001. Three samples of NP did not show amplification. Trends are similar to those seen by NanoString. CXCL9 and IDO-1, however, did not show statistical significance as seen on NanoString. APN, acute pyelonephritis; AR, acute rejection; CXCL, CXC chemokine ligand; NP, native kidney pyelonephritis