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. 2017 Jan 23;12(1):e0169092. doi: 10.1371/journal.pone.0169092

Fig 1. TSA treatment alone improves early developmental efficiency of SCNT embryos.

Fig 1

SCNT embryos at the pronuclear stage were cultured in PZM-3 supplemented with 50 nM TSA for 24 h and embryos cultured in PZM-3 containing DMSO were served as NT control, then TSA and DMSO were washed out and embryos were cultured in fresh PZM-3 medium until day 7. The effect of HDACs inhibition on the proportion of embryos that reached the 2-cell stage within 48 h after activation (A), proportion of embryos developing to the blastocyst stage (Day 7) (B) and total cell number per blastocyst (C) are shown. The experiment was repeated at least three times with 200 embryos per group. All the percentage data are expressed relative to the number of 1-cell embryos and are shown as mean ± S.E.M. Values with different superscripts across treatments indicate significant differences (P < 0.05).