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. 2017 Jan 23;12(1):e0169092. doi: 10.1371/journal.pone.0169092

Fig 2. BIX-01294 treatment alone enhances early developmental efficiency of SCNT embryos.

Fig 2

SCNT embryos at the pronuclear stage were cultured in PZM-3 supplemented with different concentrations of BIX-01294 at 0 (embryo culture water as control), 0.25, 0.5, 1.0 and 2.0 μM for 24 h, then BIX-01294 in different treatment groups were washed out and embryos were cultured in fresh PZM-3 medium until day 7. The effect of G9A inhibition on the proportion of embryos that reached the 2-cell stage within 48 h after activation (A), proportion of embryos developing to the blastocyst stage (Day 7) (B) and total cell number per blastocyst (C) are shown. The experiment was repeated at least three times with 130 embryos per group. All the percentage data are expressed relative to the number of 1-cell embryos and are shown as mean ± S.E.M. Values with different superscripts across treatments indicate significant differences (P < 0.05).