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. 2017 Jan 23;12(1):e0169092. doi: 10.1371/journal.pone.0169092

Fig 3. TSA and BIX-01294 treatment combined enhances early developmental efficiency of SCNT embryos.

Fig 3

SCNT embryos at the pronuclear stage were cultured in PZM-3 supplemented with 50 nM TSA or 50 nM TSA and 1 μM BIX-01294 for 24 h and embryos cultured in PZM-3 containing DMSO were served as NT control, then DMSO, TSA and BIX-01294 in different groups were washed out and embryos were cultured in fresh PZM-3 medium until day7. The effect of HDACs and G9A inhibition on the proportion of embryos that reached the 2-cell stage within 48 h after activation (A), proportion of embryos developing to the blastocyst stage (Day 7) (B) and total cell number per blastocyst (C) are shown. The experiment was repeated at least three times with 220 embryos per group. All the percentage data are expressed relative to the number of 1-cell embryos and are shown as mean ± S.E.M. Values with different superscripts across treatments indicate significant differences (P < 0.05). (D) Representative images of SCNT blastocysts from different groups under bright field. DNA was labeled with propidium iodide (red). Scale bars = 50μm.