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. 2017 Jan 23;12(1):e0169905. doi: 10.1371/journal.pone.0169905

Fig 4. SSO-FRET-FLIM of BRI1-GFP and BRI1-GFP/SERK3/BAK1-mCherry in absence and presence of BL.

Fig 4

(A) SSO fluorescence intensity image of BRI1-GFP expressed in root epidermal cells pretreated with 50 μM BRZ (B) SSO fluorescence lifetime image of BRI1-GFP expressed in root epidermal cells pretreated with 50 μM BRZ (C) SSO fluorescence lifetime image of BRI1-GFP/SERK3/BAK1-mCherry expressed in root epidermal cells pretreated with 50 μM BRZ. (D) SSO fluorescence lifetime image of BRI1-GFP/SERK3/BAK1-mCherry expressed in root epidermal cells pretreated with 50 μM BRZ and subsequent incubation with 1 μM BL for 1 hour. The color bar represents the false color code for fluorescence lifetime (τ) distribution. Scale bar represents 10 μm.