Table 2. Quantification of clusters/ μm² and cluster size of BRI1 and SERK3.

		BRZ	BL after BRZ pre-treatment	No treatment
BRI1-GFPline 1	clusters/ μm2	0.55 ± 0.13	0.49 ± 0.08	0.59 ± 0.1
	cluster size (μm2)	0.29 ± 0.04	0.31 ± 0.04	0.29 ± 0.02
BRI1-GFP line 2	clusters/ μm2	0.54± 0.14	0.60 ± 0.14	0.59 ± 0.14
	cluster size (μm2)	0.45 ± 0.06	0.49 ± 0.06	0.41 ± 0.05
SERK3-mCherry	clusters/ μm2	1.05 ± 0.19	0.75 ± 0.14	0.83 ± 0.11
	cluster size (μm2)	0.12 ± 0.02	0.08 ± 0.03	0.13 ± 0.02

Quantification of nanocluster density and size of BRI1-GFP and SERK3/BAK1-mCherry of 5 day old Arabidopsis seedlings showing the average number of nanoclusters per μm² and average cluster size in μm² upon different treatments using VAEM. 5 μM BRZ was added to growth media for 3 days prior to ligand application. Ligand application was accomplished by incubation of seedlings with 1 μM BL for 1h. All experiments were performed twice; three images were collected for three seedlings per experiment (n = 9). Numbers are given with their respective pooled standard error of the mean (SEM).