Table 2. Cellulosomal endoglucanases with significantly different levels when comparing Tissue and Whatman Paper incubations.
| Pellet | Supernatant | |||||||
|---|---|---|---|---|---|---|---|---|
| Gene ID | Protein/Gene name | Substratea) | Interactionb) | Substratea) | Interactionb) | Modular structurec) | Localizationd) | Protein function or namee) |
| Ccel_1648 | β-glucanase R, Cel9R | -0.24 | +0.29 | S-GH9-CBM3-UNK-CBM3-DOC1 | cellulosome | Endoglucanase Cel9R | ||
| Ccel_0732* | Cel9E | -0.26 | S-UNK-CBM4-UNK-GH9-UNK-DOC1 | cellulosome | Endoglucanase/cellobiohydrolase Cel9E | |||
| Ccel_0734* | Cel9H | -0.18 | S-UNK-GH9-UNK-CBM3-DOC1 | cellulosome | Endoglucanase Cel9H | |||
| Ccel_1249 | β-glucanase T, Cel9T | -0.25 | S-GH9-CBM3-DOC1 | cellulosome | Endoglucanase Cel9T | |||
| Ccel_0735* | Cel9J | -0.42 | -0.38 | S-GH9-CBM3-DOC1 | cellulosome | Endoglucanase Cel9J | ||
| Ccel_0740* | Cel5N | -0.28 | -0.25 | S-GH5-DOC1 | cellulosome | Endoglucanase Cel5N | ||
| Ccel_0753 | Cel9P, P90 | -0.22 | -0.28 | S-GH9-CBM3-UNK-DOC1 | cellulosome | Endoglucanase Cel9P | ||
| Ccel_0755 | cellulase U / cellulase S, Cel9U | -0.13 | S-UNK-GH9-DOC1 | cellulosome | Endoglucanase Cel9U | |||
| Ccel_2392 | Cel9V | -0.21 | S-UNK-CBM4-UNK-GH9-DOC1 | cellulosome | Endoglucanase/cellobiohydrolase Cel9V | |||
| Ccel_1099 | celCCA, Cel5A, Cca | -3.63 | -3.63 | S-GH5-DOC1 | cellulosome | Endoglucanase/endoxylanase Cel5A | ||
| Ccel_2337 | CMCase, P66 | +0.28 | S-GH5-DOC1 | cellulosome | Endoglucanase | |||
| Ccel_0429 | P99 | +0.40 | S-GH44-DOC1-UNK-CBM44 | cellulosome | Endoglucanase Cel44O (PKD domain containing protein) | |||
The proteins are listed according to the observed effects and to their function. The statistical models take into account the replicates and their variability.
* in the first left column (“Gene ID”) indicate genes encoded in the “cip-cel” gene cluster, which codes for 12 key cellulosomal components.
a) The log10 fold change values are indicated for the proteins with statistically significant substrate effects (Q-value < = 0.01). Tissue incubations are used as a reference (positive values when the protein levels are higher in the Tissue incubations).
b) The log10 fold change values are indicated for the proteins with statistically significant substrate-by-time interaction effects (Q-value < = 0.01). Tissue incubations are used as a reference (positive values when the protein levels increase faster or decrease slower in the Tissue incubations).
c) According to [16, 21] and the present study: S: signal sequence; GH: family of glycoside hydrolase; PL: family of pectate lyase; CE: family of carbohydrate esterase; GT: family of glycosyl transferase; CBM: family of carbohydrate-binding module; DOC1: dockerin type 1 module; COH: cohesin type I module, LNK: linker sequence; SLH: surface-layer homology sequence; COG: clusters of orthologous groups; UNK: unknown function module or sequence; TSP_C: thrombospondin C-terminal region; fn3: fibronectin type III domain.
d) Known (in bold) or predicted localization.
e) Predicted or characterized (in bold) activities or protein names according to [16] and to UniprotKB database.