Fig. 4.

Bco1 loss alters mRNA expression of steroidogenic enzymes in the testes. Testicular cholesterol (A), mRNA expression of cholesterol esterification enzymes (B), and mRNA and protein expression of steroidogenic enzymes (C) were measured. Lcat, Soat1, Soat2, Hmgcr, Scarb1, Star, Cyp17a1, Hsd17b3, and Hsd17b2 mRNA expression was determined by qPCR and HMG-CoA reductase and CYP11A1 protein expression was measured by Western blot analysis. mRNA expression was normalized to Rpl19, and protein expression was normalized to GAPDH. Data are presented as means ± SE; n = 8–18, *P < 0.05, ***P < 0.001.