Table 1.
Genes differentially regulated in prostates of Bco1−/− mice
| Symbol | Gene Name | Gene Ontology Process | Fold Change | P Value |
|---|---|---|---|---|
| Cluster 1 | ||||
| E2f1 | E2F transcription factor 1 | Cell cycle regulation | 0.62 | 0.0708 |
| Ccne2 | Cyclin E2 | Cell cycle regulation | 0.79 | 0.0291 |
| Foxm1 | Forkhead box M1 | Transcription factor | 0.57 | 0.0157 |
| Jun | Jun proto-oncogene (AP-1) | Cell cycle regulation | 0.82 | 0.0484 |
| Bax | BCL2-associated X protein | Apoptosis | 0.82 | 0.0030 |
| Erbb1 | Epidermal growth factor receptor | Proliferation; apoptosis | 0.86 | 0.0376 |
| Mcl1 | Myeloid cell leukemia sequence 1 | Apoptosis | 0.90 | 0.0146 |
| Vegfb | Vascular endothelial growth factor B | Growth factor | 0.87 | 0.0179 |
| Prom1 | Prominin 1 (Cd133) | Proliferation; stem cell marker | 0.72 | 0.0006 |
| Tgfb2 | Transforming growth factor, β2 | Proliferation; apoptosis | 0.63 | <0.0001 |
| Kras | Kirsten rat sarcoma viral oncogene | Intracellular signaling | 0.53 | <0.0001 |
| Pcna | Proliferating cell nuclear antigen | Proliferation | 0.88 | 0.0027 |
| Ki67 | Marker of proliferation Ki-67 | Proliferation | 0.56 | 0.0025 |
| Aurkb | Aurora kinase B | Cell cycle regulation | 0.46 | 0.0005 |
| Aurka | Aurora kinase A | Cell cycle regulation | 0.68 | 0.0449 |
| Birc5 | Survivin | Apoptosis | 0.60 | 0.0154 |
| Ccnb2 | Cyclin B2 | Cell cycle regulation | 0.49 | 0.0021 |
| Casp3 | Caspase 3 | Apoptosis | 0.93 | 0.0320 |
| Cdc25c | Cell division cycle 25C | Cell cycle regulation | 0.63 | 0.1108 |
| Cluster 2 | ||||
| Vegfa | Vascular endothelial growth factor A | Angiogenesis | 1.51 | <0.0001 |
| Met | HGF/SF receptor | Growth factor receptor | 1.66 | <0.0001 |
| Gpx3 | Glutathione peroxidase 3 | Catalytic activity | 3.06 | <0.0001 |
| Bco1 | β-Carotene oxygenase 1 | Catalytic activity | 8.21 | 0.0001 |
| Cluster 3 | ||||
| Esr2 | Estrogen receptor 2 (β) | Nuclear hormone receptor | 1.60 | 0.0016 |
| Akt2 | Protein kinase Bβ | Cell cycle regulation; apoptosis | 1.23 | 0.0276 |
| Erbb2 | Her2/Neu receptor | Proliferation; apoptosis | 1.12 | 0.1489 |
| Krt5 | Keratin 5 | Cytoskeleton | 1.29 | 0.0943 |
| Vdr | Vitamin D receptor | Nuclear hormone receptor | 1.21 | 0.0232 |
| Igf1r | Insulin-like grwoth factor 1 receptor | Growth factor receptor | 1.25 | 0.0196 |
Bco1 disruption alters the prostatic expression of genes involved in cell cycle progression, proliferation, apoptosis, and prostate carcinogenesis. Clusters consist of significantly modulated (P < 0.005) genes and other genes that were correlated (R2 ≥ 0.93) with their expression. These gene clusters are also highlighted in Fig. 7A. Gene names and symbols were obtained from the Online Mendelian Inheritance in Man (OMIM) database. Cellular/molecular process classification was done according to gene ontology annotations in the PANTHER Classification System. P values ≤ 0.005 are in bold.