P2X7RKO mice have altered brain mRNA expressions before and after sleep deprivation. Relative fold-changes (+SE) in IL-1β, TNF-α, brain-derived neurotrophic factor (BDNF), adenosine deaminase (ADA), and P2X4R mRNA in the hypothalamus, somatosensory cortex, and hippocampus between WT and P2X7RKO (KO) with spontaneous sleep (Control) or after 6 h of sleep deprivation (6SD) are shown. *P < 0.05, **P < 0.01, ***P < 0.001 for treatment-induced differences. #P < 0.05, ##P < 0.01, ###P < 0.001 for strain differences.