Skip to main content
. 2016 Oct 5;311(6):R1125–R1134. doi: 10.1152/ajpregu.00032.2016

Fig. 5.

Fig. 5.

Impact of shCLOCK on expression of cell cycle regulators. WT and shClock-3 cells were plated in media with 10% serum, serum starved to synchronize cells, and then media with 10% serum + EGF were added (time 0). Cells were collected every 4 h over 48 h. RNA was isolated from cultures collected during the 8- to 32-h period, and Q-PCR analysis was used to measure steady-state CCND1 (A), WEE1 (B) and TP63 mRNA levels. Two-way ANOVA of data from three replicate experiments showed that time and line (WT vs. shClock-3) had significant (P < 0.001) impacts on CCND1 and WEE1 expression, and line had a significant (P < 0.001) impact of TP63 expression.