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. 2016 Oct 5;311(6):R1125–R1134. doi: 10.1152/ajpregu.00032.2016

Fig. 6.

Fig. 6.

Impact of shCLOCK on markers of differentiation in HC11 cells. WT, scramble, and shClock-3 cells were plated and grown to confluence in 10% + EGF, media were changed to 10% serum alone and undifferentiated (undiff) cultures were collected after 48 h. Differentiated (diff) cultures were incubated for an additional 96 h in media supplemented with dexamethasone, Ins, and prolactin + 10% serum. RNA was isolated, and qPCR analysis was used to measure steady-state CSN2 (A), FASN (B), and CDH1 (C), mRNA levels. Two-way ANOVA of three replicate experiments showed that state of differentiation had a significant impact on CDH1, FASN, and CSN2 expression and line (WT HC11, scramble, shClock-3) had significant (P < 0.05) effect on CDH1 and FASN expression. Tukey post hoc analysis demonstrated that expression level was different from HC11-undiff (*P < 0.05), or from HC11-diff (aP < 0.05).